Open Wednesday – Saturday, 11am-4pm

Please visit our COVID-19 Safety Guidelines page to see what is expected of our visitors and what we are doing to ensure your safety.

Our museum complex is located in downtown Gainesville at 513 East University Avenue next to Sweetwater Branch. Tour information can be found below.


FREE (donations encouraged)


Parking is available in the museum’s parking lot and on the evenings and weekends next door at the Law Offices of Attorneys Folds & Walker and at the Alachua County Public Schools District Office.

There is one wheelchair accessible parking space in the museum’s parking lot and one behind the 1867 Matheson House.


The museum, Library & Archives, and 1867 Matheson House have an ADA accessible ramp.

The museum and Library & Archives also have ADA accessible restrooms.

The Tison Tool Barn, which is housed in a historic barn, is not ADA accessible but it is currently closed to the public, except by appointment.


Tours of the current exhibit are available free of charge. We are offering a limited number of tours of the 1867 Matheson House to no more than 3 people at a time. Please call 352-378-2280 or email to schedule a house tour. It is possible that we can accommodate unscheduled tours during the museum’s open hours, but not guaranteed.

Third and Fourth Graders Touring the 1867 Matheson House

Gift Shop

The museum gift shop is filled with historic postcards, yearbooks, maps, and an excellent collection of books on Florida history and by local authors. Members receive a 20% discount. Follow us on social media or sign up for our email newsletter to receive notification of sales. We accept cash, checks, and all major credit cards.


Our research library and archives are located across the street in the Matheson Library & Archives at 418 East University Avenue and is open by appointment only. The museum’s library books are catalogued in the collection of the Alachua County Library District and are searchable on their website. For information on making a research appointment please visit our Library & Archives page.